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KMID : 0665220100230010030
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Korean Journal of Food and Nutrition
2010 Volume.23 No. 1 p.30 ~ p.38
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The Effects of Vitamin C on the Activity of Liver Enzymes and Hepatic Damage in Rats Treated with Radiation and Aflatoxin B1
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Kang Jin-Soon
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Abstract
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This study was conducted to determine the effects of vitamin C on the activity of liver function enzymes and electromicrographic changes in white rats treated with aflatoxin or X-ray and . Six week-old male Sprague-Dawley rats were randomly divided into five groups: a control group, treated group, treated group with vitamin C, X-ray and co-treated group, X-ray and co-treated group with vitamin C. On the first day of the experiment, only one dose of X-rays was exposed to the entire liver at 1,500 cGy. Next, vitamin C was injected at 10 mg/kg body weight by intraperitoneal injection, followed 1 hr later by the administration of 0.4 mg/kg of by intraperitoneal injection. These treatments were then administered every three days over a period of 15 days. On the 16th day of treatments, the animals were sacrificed. Analysis of the activity of the liver function enzymes, GOT, ALK phatase and LDH, in the sera of rats revealed that they were somewhat increased by treatment, X-ray and co-treatment when compared to the control group. Furthermore, the activity of these enzymes decreased in response to administration of vitamin C. Especially, the levels of GOT were remarkably decreased in the treated group treated with vitamin C when compared to the group treated with alone(p<0.001). Electromicrographic analysis revealed cloudy swelling, necrosis, vesicular degeneration and fat accumulation of hepatocytes in response to treatment with or co-treatment with X-ray and . However, the destruction of hepatic cells was considerably lower in the vitamin C-treated group. These results indicate that vitamin C had ameliorating effects on the hepatic cell damage.
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KEYWORD
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vitamin C, aflatoxin B1, radiation, hepatic cell damage
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